Coding

Part:BBa_K5063009:Design

Designed by: Wong Chung Kiu   Group: iGEM24_HK-United   (2024-09-28)


TP1_P5_GFP fusion protein coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1323
    Illegal BamHI site found at 1504
    Illegal XhoI site found at 604
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added the GFP to check the presence of the fusion protein for easier detection


Source

[1] Leong, D. P., Joseph, P. G., McKee, M., Anand, S. S., Teo, K. K., Schwalm, J. D., & Yusuf, S. (2017). Reducing the global burden of cardiovascular disease, part 2: prevention and treatment of cardiovascular disease. Circulation research, 121(6), 695-710.https://www.ahajournals.org/doi/epub/10.1161/CIRCRESAHA.117.311849 [2] Loo, S., Kam, A., Dutta, B., Zhang, X., Feng, N., Sze, S. K., ... & Tam, J. P. (2024). Broad-spectrum ginsentides are principal bioactives in unraveling the cure-all effects of ginseng. Acta Pharmaceutica Sinica B, 14(2), 653-666.https://www.sciencedirect.com/science/article/pii/S2211383523004227 [3] Dutta B, Loo S, Kam A, Sze SK, Tam JP. Ginsentide TP1 Protects Hypoxia-Induced Dysfunction and ER Stress-Linked Apoptosis. Cells. 2023 May 16;12(10):1401. doi: 10.3390/cells12101401. PMID: 37408235; PMCID: PMC10216702.

Lammi C, Fassi EMA, Li J, Bartolomei M, Benigno G, Roda G, Arnoldi A, Grazioso G. Computational Design and Biological Evaluation of Analogs of Lupin Peptide P5 Endowed with Dual PCSK9/HMG-CoAR Inhibiting Activity. Pharmaceutics. 2022 Mar 18;14(3):665. doi: 10.3390/pharmaceutics14030665. PMID: 35336039; PMCID: PMC8951016.

References